ORQUITE POR STREPTOCOCCUS SUIS EM SUÍNO – RELATO DE CASO

Autores

  • Mariana Iara Magalhães
  • Isadora Fernanda Pelaquim
  • Isabela Leite Doretto
  • Nubia Camargo Callegarette
  • Camila Dias Porto
  • Alessandre Hataka

Resumo

O Streptococcus suis é uma bactéria gram positiva com mais de 35 sorotipos. No Brasil, o sorotipo 2 é o mais prevalente. O stress é um item importante e pode precipitar a infecção. A mortalidade dos animais acometidos pode variar entre quatro a 14 % e a susceptibilidade diminui com o aumento da idade. Entre os principais sinais clínicos da enfermidade, destaca-se otite interna, incoordenação motora, problemas neurológicos, septicemia, pneumonia, endocardite, rinite e principalmente meningite. A identificação do agente é feita através de isolamento, testes sorológicos e parâmetros bioquímicos. Um suíno sem raça definida, macho, de quatro anos de idade, com aumento de bolsa escrotal e secreção escura no ouvido foi encaminhado ao HV da UNIMAR para atendimento. Ao exame clínico o animal apresentava aumento da bolsa escrotal, anorexia, apatia, ulcerações na pele no membro posterior esquerdo, vômito esverdeado, olhos com mucosas congestas. Após atendimento clínico o animal foi a óbito e realizou-se a necropsia, foram colhidas amostras para exames histopatológico e microbiológico do testículo, líquido peritoneal e do exsudato da bolsa escrotal. No exame interno apresentava extenso flegmão e quantidade acentuada de líquido fibrinopurulento amarronzado na cavidade abdominal, pleuropneumonia severa, megaureter bilateral e cistite severa. Os resultados da cultura em ágar sangue de carneiro e BHI evidenciaram o crescimento de coco bacilo Gram positivo, no ágar sangue suas colônias eram puntiformes, translúcidas e delicadas, não mucóides de bordas arredondadas e não hemolíticas em 24 horas. Os testes para amilase, hidrólise da esculina, OF (glicose) tiveram resultados positivos. Ambas amostras apresentaram resistência a optoquina. Os resultados das provas de fermentação por 14 dias foram: positividade para glicose, sacarose, lactose, maltose, trealose, frutose, galactose, xilose e glicerol. E resultado negativo para sorbitol e manitol. Com base nos resultados acima concluiu-se tratar de septicemia por Streptococcus suis.

 

Referências

AARESTRUP, F.M.; JORSAL, S.E.; JENSEN, N.E. Serological characterization and antimicrobial susceptibility of Streptococcus suis isolates form diagnostic samples in Denmark during 1995 and 1996. Veterinary Microbiology, v. 60, n.1, p. 59-66, 1998.

AMASS, S.F.; SANMIGUEL, P.; CLARK, L.K. Demonstration of vertical transmission of Streptococcus suis in Swine by genomic fingerprinting. Journal of Clinical Microbiology, v. 35, n. 6, p. 1595-1596, 1997.

ARENDS, J.P.; ZANEN, H.C. Meningitis caused by Streptococcus suis in humans. Reviews of Infectious Diseases, v.10, n. 2, p. 131-137, 1988.

BARCELLOS, D.E.S.N.; BOROWSKI, S.M.; OLIVEIRA, S.J. Infecção de suínos pelo Streptococcus suis tipo II no Rio Grande do Sul: pesquisa de portadores pelo exame bacteriológico de amígdalas coletadas em frigoríficos. Arquivo da Faculdade de Veterinária, UFRGS, v. 23, p. 101-107, 1995.

BERTHELOT-HERAULT, F.; GOTTSCHALK, M.; MORVAN, H.; KOBISCH, M. Dilemma of virulence of Streptococcus suis: Canadian isolate 89-1591 characterized as a virulent strain using a standardized experimental model in pigs. Canadian Journal Veterinary Research, v. 69, p. 236–40, 2005.

BOYE, M.; FEENSTRA, A.A.; TEGMEIER, C.; ANDRESEN, L.O.; RASMUSSEN, S.R.; BILLE-HANSEN, V. Detection of Streptococcus suis by in situ hybridization, indirect immunofluorescence, and peroxidase-antiperoxidase assays in formalin-fixed, paraffin-embedded tissue sections from pigs. Journal of Veterinary Diagnostic Investigation, v. 12, n. 3, p. 224-232, 2000.

CHARLAND, N.; NIZET, V.; RUBENS, C.E.; KIM, K.S.; LACOUTURE, S.; GOTTSCHALK, M. Streptococcus suis serotype 2 interactions with human brain microvascular encothelial cells. Infection and Immunity, v. 68, n. 2, p. 637-643, 2000.

CHATELLIER, S.; HAREL, J.; ZHANG, Y.; GOTTSHALK, M.; HIGGINS, R.; DEVRIESE, L. A.; BROUSSEAU, R. Phylogenetic diversity of Streptococcus suis strains of various serotypes as revealed by 16S rRNA gene sequence comparison. International Journal Systematic Bacteriology, v. 48, n. 2, p. 581-589, 1998.

CHRISTENSEN, P.; KAHLMETER, G.; JONSSON, S.; KRONVALL, G. New method for the serological grouping of Streptococci with specific antibodies adsorbed to protein A-containing Staphylococci. Infection and Immunity, v.7, n.6, p.881–5, 1973.

CORRY, J.E.; HINTON, M.H. Zoonoses in the meat industry: a review. Acta Vetetinaria Hungarica, v. 45, p. 457-79, 1997.

DE MOOR, C.E. Septicemic infections in pigs caused by haemolytic streptococci of new Lancefield groups designated R, S and T. Antonie van Leeuvenhoek, v. 29, n. 2, p. 272-280, 1963.

DEE, S.A.; CARLSON, A.R.; WINKELMAN, N.L.; COREY, M.M. Effect of management practices on the Streptococcus suis carrier rate in nursery swine. Journal of the American Veterinary Medical Association, v. 2003, n. 2, p. 295-299, 1993.

DEVRIESE, L.A.; CEYSSENS, K.; HOMMEZ, J.; KILPPER-BALZ, R.; SHLEIFER, K.H. Characteristics of different Streptococcus suis ecovars and description of a simplified identification method. Veterinary Microbiology, v. 26, n. 1-2, p. 141-150, 1991.

DRITZ, S.S.; CHENGAPPA, M.M.; NELSSEN, J.L.; TOKACH, M.D.; GOODBAND, R.D.; NIETFELD, J.C.; STAATS, J.J. Growth and microbial flora of nonmedicated segregated, early weaned pigs, from a commercial swine operation. Journal of the American Veterinary Medical Association, v. 208, n. 5, p. 711-715, 1996a.

DRITZ, S.S.; OWEN, K.Q.; GOODBAND, R.D.; NELSSEN, J.L.; TOKACH, M.D.; CHENGAPPA, M.M.; BLECHA, F. Influence of lipopolysaccharide-induced immune challenge and diet complezity on growth performance and acutephase protein production in segregated early-weaned pigs. Journal of Animal Science, v.74, n. 7, p. 1620-1628, 1996b.

FACKLAM, R. What happened to the streptococci: overview of taxonomic and nomenclature changes. Clinical Microbiology Reviews, v. 15, p. 613-630, 2002.

FRANÇOIS, B.; GISSOT, V.; PLOY, M.C.; VIGNON, V. Recurrent septic shock due to Streptococcus suis. Journal of Clinical Microbiology, v. 36, n. 8, p. 2395, 1998.

GALINA, L.; VECHT, U.; WISSELINK, H.J.; PIJOAN, C. Prevalence of various phenotypes of Streptococcus suis isolated from swine in the U. S. A. based on the presence of muramidase-released protein and extracellular factor. Canadian Journal Veterinary Research., v. 60, n. 1, p. 72-74, 1996.

GOGOLEWSKI, R.P.; COOK, R.W.; O’CONNELL, C.J. Streptococcus suis serotypes associated with disease in weaned pigs. Australian Veterinary Journal, v. 67, n. 6, p. 202-204, 1990.

GOTTSCHALK, M.; LEBRUN, A.; WISSELINK, H.; DUBREUIL, D.; SMITH, H.; VECHT, U. Production of virulencerelated proteins by Canadian strains of Streptococcus suis capsular type 2. Canadian Journal Veterinary Research., v. 62, n. 1, p. 75-79, 1998.

GOTTSCHALK, M.; SEGURA, M.; The patogenesis of meningitis caused by Streptococcus suis: the unresolved questions. Veterinary Microbiology, v. 76, n. 3, p. 259-272, 2000.

HASHIKAWA, S.; IINUMA, Y.; FURUSHITA, M.; OHKURA, T.; NADA, T.; TORII, K. et al. Characterization of group C and G streptococcal strains that cause streptococcal toxic shock syndrome. Journal of Clinical Microbiology, v. 42, p. 186–92, 2004.

HIGGINS, R.; GOTTSCHALK, M. Streptococcal disease. In: STRAW, B.E.; D’ALLAIRE, S.; MENGELING, W.L.; TAYLOR, D.J. Disease of swine. Ames: Iowa State University Press, p. 563-570, 1999.

HUANG, Y.T.; TENG, L.J.; HO, S.W.; HSUEH, P.R. Streptococcus suis infection. Journal of Microbiology, Immunology and Infection, v. 38, p. 306–13, 2005.

JACOBS, A.A.C.; VAN DEN BERG, A.J.G.; LOEFFEN, P.L.W. Protection of experimentally infected pigs by suilisyn, the thiol-activated haemolysin of Streptococcus suis. Veterinary Record, v. 7, n. 139, p. 225-228, 1996.

KRAUSS, H.; WEBER, A.; APPEL, M. Editors. Zoonoses—infectious diseases transmissible from animals to humans, 3rd ed. Washington: ASM Press; p. 239-40, 2003.

LEE, G.T.; CHIU, C.Y.; HALLER, B.L.; DENN, P.M.; HALL, C.S.; GERBERDING, J.L. Streptococcus suis meningitis, United States. Emerging Infectious Diseases. v. 14, p. 183–5, 2008.

LUN, Z.R.; WANG, Q.P.; CHEN, X.G. et al. Streptococcus suis: an emerging zoonotic pathogen. Lancet Infectious Diseases, v. 7, p. 201-209, 2007.

LUQUE, I.; TARRADAS, C.; ARENAS, A.; MALDONADO, A.; ASTORGA, R.; PEREA, A. Streptococcus suis serotypes associated with different disease conditions in pigs. The Veterinary Record, v. 27, n. 142, p. 726-727, 1998.

MAROIS, C.; BOUGEARD, S.; GOTTSCHALK, M.; KOBISCH, M. Multiplex PCR assay for detection of Streptococcus suis species and serotypes 2 and 1/2 in tonsils of live and dead pigs. Journal of Clinical Microbiology. v. 47, p. 3169–75, 2004.

OKWUMABUA, O.; STAATS, J.; CHENGAPPA, M. M. Detection of genomic heterogeneity in Streptococcus suis isolates by DNA restriction fragment length polymorphisms of rRNA genes (ribotyping). Journal Clinical Microbiology, v. 33, n. 4, p. 968-972, 1995.

PAYVANDI, F.; SRIKUMARAN, S.; SHIELDS, T. R.; ERICKSON, E. D. Monoclonal antibodies for coaglutination of Streptococcus suis type 1. Veterinary Microbiology, n. 20, p. 349-356, 1989.

PICARD, F.J.; KE, D.; BOUDREAU, D.K.; BOISSINOT, M.; HULETSKY, A.; RICHARD, D. et al. Use of tuf sequences for genus-specific PCR detection and phylogenetic analysis of 28 streptococcal species. Journal of Clinical Microbiology, v. 42, p. 3686–95, 2004.

ROBERTS, M.C. Update on acquired tetracycline resistance genes. FEMS Microbiology Letters, v. 245, p. 195–203, 2005.

SANFORD, S.E.; HIGGINS, R. Streptococcal disease. In: LEMAN, A. D. Disease of swine. Ames: Iowa, State University Press, p. 588-598, 1992.

SANFORD, S.E.; HIGGINS, R. Streptococcal diseases. In: L, A.D.; STRAW, B.E.; MENGELING, W.L.; D’ALLAIRE, S.; TAYLOR, D.J. Diseases of Swine. Iowa State University Press, Ames, Iowa, p. 588-590, 1992.

SANTOS, J.L.; DEL’ARCO, A.E.; RIBEIRO, M.C.E.; GUIMARÃES, W. Ocurrence of Streptococcus suis serotypes in pigs in Brazil. In: THE 16TH INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 17, 2000, Melborne. Proceedings... Austrália, p. 536, 2000.

SEPULVEDA, E.M.C.; ALTMAN, E.; KOBISCH, M.; ALLAIRE, SD.; GOTTSCHALK, M. Detection of antibodies against Streptococcus suis capsular type 2 using a purified polysaccharide antigen-based indirect ELISA. Veterinary Microbiology, v. 52, n. 1-2, p. 113-125, 1996.

STAATS, J.J.; FEDER, I.; OKWUMABUA, O.; CHENGAPPA, M.M. Streptococcus suis: past and present. Veterinary Research Communications, v. 21, n. 6, p. 381-407, 1997.

STAATS, J.J.; PLATTNER, B.L.; NIETFELD, J.; DRITZ, S.; CHENGAPPA, M.M. Use of ribotyping and hemolysin activity to identify highly virulent Streptococcus suis type 2 isolates. Journal of Clinical Microbiology, v. 36, n. 1, p. 15-19, 1998.

*SUANKRATAY, C.; INTALAPAPORN, P.; NUNTHAPISUD, P.; ANMYINGMONGKOL, K.; WILDE, H. Streptococcus suis meningitis in Thailand. Southeast Asian. Journal of Tropical Medicine and Public Health. v. 35, p. 868–76, 2004.

TORREMORELL, M.; PIJOAN, C. Prolonged persistence of an epidemic Streptococcus suis strain in a closed pig population. Veterinary Record, v. 143, n. 14, p. 394-395, 1998.

VECHT, U.; WISSELINK, H.J.; STOCKHOFE-ZURWIEDEN, N.; SMITH, H.E. Characterization of virulence of the Streptococcus suis serotype 2 reference strain Henrichsen S 735 in newborn gnobiotic pigs. Veterinary Microbiology, v. 51, n.1-2, p. 125-136, 1996.

YE, C.; ZHU, X.; JING, H.; DU, H.; SEGURA, M.; ZHENG, H. et al. Streptococcus suis sequence type 7 outbreak, Sichuan, China. Emerging Infectious Diseases. V. 12, p. 1203–8, 2006.

*YU, H. et al. Human Streptococcus suis outbreak, Sichuan, China. Emerging Infectious Diseases, 2006, Jun; 12. (http://www.cdc.gov/ncidod/eid/vol12no06/05-1194.htm).

WILLIAMS, N.H.; STAHLY, T.S.; ZIMMERMAN, D.R. Effect of chronic immune system activation on body nitrogen retention, partial efficiency of lysine utilization, and lysine needs of pigs. Journal of Animal Science, v. 75, n. 9, p. 2472-2480, 1997a.

WILLIAMS, N.H.; STAHLY, T.S.; ZIMMERMAN, D.R. Effect of level of chronic immune system activation on the growth and dietary lysine needs of pigs fed from 6 to 112 kg. Journal of Animal Science, v. 75, n. 9, p. 2481-2496, 1997b.

WISSELINK, H.J.; REEK, F.H.; VECHT, U.; STOCKHOFE-ZURWIEDEN, N.; SMITS, M.A.; SMITH, H. E. Detection of virulent strains of Streptococcus suis type 2 and highly virulent strains of Streptococcus suis. type 1 in tonsillar specimens of pigs by PCR. Veterinary Microbiology, v. 67, n. 2, p. 143-157, 1999.

WISSELINK, H.J.; SMITH, H.E.; STOCKHOFE-ZURWIEDEN, N.; PEPERKAMP, K.; VECHT, U. Distribution of capsular types and production of muramidase released protein (MRP) and extracellular factor (EF) of Streptococcus suis strains isolated from diseased pigs in seven European countries, Veterinary Microbiology, v. 74, n. 3, p. 237-248, 2000.

Downloads

Publicado

2021-12-19

Edição

Seção

Artigos